http://doi.org/10.33698/NRF0263-Davinder Kaur, Surinder Jaspal, Sukhminderjit Singh BajwaAbstract
Introduction: Central venous catheters (CVCs) are inserted to critically ill patients admitted in intensive care units for hemodynamic monitoring and administration of medications and nutrition. Objectives: To assess the bacteriological prole among critically ill patients with open versus closed central venous catheter access system. Methodology: The present study was carried out as a randomized, prospective, parallel group design. The ethical clearance was obtained from Institute Ethics Committee of Gian Sagar Medical College and Hospital, Ram Nagar. All the patients admitted in the Intensive Care Unit (ICU) and needed central line catheter were included in study. Patients with any positive culture of urine, surgical drains uid, respiratory secretions and or evidence of positive culture like wound swab culture, pleural uid etc. were excluded from study. Patients were randomized into two groups (200 in each group) by using sequentially numbered, sealed, opaque envelopes. Training protocol and observational checklist for nurses regarding CVC care was prepared and validated. Written informed consent was taken from relatives of patients. Data was collected from patients by using socio-demographic and clinical prole sheet and data related to CVC such as date of CVC insertion, type and site of CVC insertion, signs of local and systemic infection was done till the termination of CVC. In experimental group closed catheter access system (Luer-access split septum) was used while in control group, open access system -three way system was used. If any patient developed fever, blood culture, urine culture and tracheal secretions were sent for culture and sensitivity. Collected data was analyzed for descriptive and inferential statistics. Results: In experimental group 37.1% had positive blood culture whereas in control group all the patients had positive blood culture. This shows the signicant impact of intervention i.e. the prevalence of positive blood culture was signicantly lesser in experimental group than control group(X2-58.305, p= 0.0001). Gram -ve bacilli E.coli and Klebsiella pneumoniae was present in both groups in the same proportion. Closed catheter access system (Luer access split septum) had better efcacy in prevention of catheter related blood stream infection.
Key Words: Catheter related blood stream infection (CRBSI); Luer access split septum connectors; Three way connectors; Prevention of CRBSI
Prof Davinder Kaur
Principal, Gian Sagar College of Nursing, Ram Nagar
Globally among hospitalized patients more that 1.4 Million suffer from complications associated with hospital acquired infections (HAI) and 7-12% acquires HAI at any given time. 1 Catheter related bloodstream infections (CRBSI), ventilator-associated pneumonia (VAP) , Catheter associated urinary tract infection (CAUTI), and surgical site infection (SSI) are most important and frequently occurring infections among admitted patients.2,3 Blood stream infections account for 14% of the hospital acquired infections and Central venous catheters (CVCs) are found to be the most common cause.4For the administration of l i fe supportive medications, total parenteral nutrition, hemodynamic monitoring and collection of blood samples CVCs are inserted to critically ill patients admitted in intensive care units.5,6Pathogenic microorganisms can gain entry into bloodstream at the time of insertion of the catheter due to contact between organism with its external surfaces. Contamination of catheter hub, administration of contaminated uids, and contamination of guide wire and handling o f i n f u s i o n l i n e s m a y l e a d t o microorganism colonization.2,7Bacterial contamination of CVC can be minimized with closed access system because the catheter connection is not open to air during the change of infusion site which s ignicantly decreases infection. 8 Researchers recommended that needleless access ports should be used on all lumens as compared to open access systems for prevention of CRBSI.9
Infection control specialists have emphasized that nurses are the key personnel for maintenance and care of venous access devices in daily clinical practice and they should be educated so that the incidence of CRBSI should be minimized. 1 0 Hence, researcher has undertaken this study with the aim to assess Bacteriological prole with open versus closed central venous catheter access system among critically ill patients admitted in Intensive Care Unit.
Material and methods
This was a randomized, prospective, parallel group, t r ial that assessed bacteriological prole among critically ill patients with open versus closed central venous catheter access system. All patients who were admitted in Intensive Care Unit (ICU) at Gian Sagar Medical College and Hospital, RamNagar, Dist Patiala, Punjab and need central line catheter were the accessible population during the period of July, 2015-February, 2017. Patients having central venous catheter at any site were included in trial. Patient with positive culture of urine, surgical drain , respiratory secretions, wound swab, pleural uid etc. were excluded from the study. Consecutive sampling technique was used for selecting sample. Sample size was 200 in each group. P a t i e n t s w e r e r a n d o m i z e d i n t o experimental and control group by using sequentially numbered, sealed, opaque envelopes. In experimental group closed catheter access system (Luer-access split septum) & in control group open access system -three-way system was used. After extensive review of l i terature and consultation with experts data collection tool was prepared which included Socio- demographic and clinical prole sheet. Tool included age, sex, diagnosis; type of CVC intervention, site of insertion and list of administered antibiotics and clinical sheet included sign of local infection, vital signs (blood pressure, pulse rate, respiratory rate and temperature), laboratory investigations (Total leukocyte count, Differential leukocyte count, blood culture, tracheal secretion culture, urine culture). Training protocol regarding care of patient with CVC and prevention of CRBSI was prepared based on standard guidelines by Centre for Diseases Control and prevention (CDC). Observational checklist was prepared to evaluate practices of nurses. Data collection tool, training protocol and observational checklist got validated by experts in eld of nursing, anesthesia and microbiology. All nurses posted in ICUs were trained for optimal care of patients with CVC before beginning of the study in their convenient time by using power point presentation and demonstration. Care practices of the staff nurses regarding care of central line were observed by using o b s e r v a t i o n a l c h e c k l i s t i n n o n – participatory observations. Permission for carrying out the study was sought from H O D o f A n e s t h e s i a a n d M e d i c a l Superintendent of GSMC&H, Ram Nagar. Ward in charges of ICU & ICCU were informed regarding study. Data was collected from ICU, ICCU of GSMC&H, Ram Nagar on all days during study period. Selection/ Screening of patients after meeting inclusions & exclusion criteria was done (420 patients were assessed for eligibility).Base line routine investigations – total leukocyte count, differential leukocyte count, blood culture, urine culture, tracheal secretion culture was done. Written consent was obtained from each enrolled patient/ their relative if patient was unconscious. Patient information sheet was lled for every patient. Patient’s details were entered in the data collection tool by researcher herself. Daily monitoring of patients done for assessment of local signs of inammation and systemic signs of infection till the CVC was in situ. If patient developed fever, then 10 ml blood was collected aseptically from peripheral vein and central venous catheter for blood culture. Urine and tracheal secretion culture was also sent. Two patients in experimental group and six patients in control group were lost to follow up due to LAMA (Leave Against Medical Advice). Collected data was analyzed by using SPSS version 20 for descriptive and inferential statistical.
In experimental group 25% patients were in age group 51-60 yrs and in control group 26 % were in age group of 61-70 yrs. Two third patients were male i.e. 66% & 57% in e x p e r i m e n t a l a n d c o n t r o l g r o u p respectively (Table 1) Both the groups were homogenous. (P >0.05 as per chi-square test).
Table 1: Demographic proﬁle of patients enrolled in both groups
|Experimental (E) group||Control group (C)|
|(n – 200) n (%)||(n – 200) n (%)||X 2 , df, p- value|
|31 – 40||19(9.5)||17(8.5)|
|41 – 50||22(11)||28(14)|
|51 – 60
61 – 70
NS – Non signicant (p>0.05)
Mean ± SD (Range) Age in years (E) 54.3 ± 18.0 (18.0 –92.0) (C) 55.9 ± 17.8 (18.0 – 100.0)
More than 70% patients have jugular line and triple lumen central venous catheter. In both groups majority patients had triple lumen CVC catheters (93.5% & 91% respectively) and very few have double lumen. Distribution of patients was found to be homogenous in both groups. (Table2) Maximum patients have cardiac diseases i.e. Coronary artery disease, Hypertension, triple vessel disease and underwent gastrointestinal surgeries in both groups. Cephalosporin and penicillin group antibiotics were administered to maximum patients in both groups.
Fig 1 depicts signs of infection faced by patients. Redness, inammation, fever and chills present in both groups but in control group more patients have signs of infection as compared to experimental group.
Table 2 : Clinical proﬁle of patients enrolled in both groups
(n – 200)
|Control group (n – 200)
|X 2 , df, p- value|
|Site of insertion||
1.345 # , 2,
|Double lumen Triple lumen||13(6.5)
# – Fisher’s exact test NS – Non signicant (p>0.05)
Fig 1: Complications faced by patients in both groups
There was no signicant difference (p.>0.05) in baseline vital parameters ( Blood pressure, pulse rate and respiratory rate ) of patients among experimental group and control group as revealed by t- test. All patients have temperature within range of 96.0 -105.8 in experimental group and 95.6- 104.2 in control group.
Table 3 depicts that 37.1% had positive blood culture and 62.9% had sterile blood culture in experimental group whereas, in control group, 100% had positive blood culture. There was highly signicant (X2- 58.305, p= 0.0001) difference in blood culture results, as an impact of intervention positive blood culture was witnessed less in experimental group than control group.
Gram-ve bacilli E.coli and Klebsiella pneumoniae were present in both groups in the same proportion. Positive urine culture and tracheal culture proportions was almost similar in both groups
Table 3 Result of blood culture among patients in both groups
|Experimental group (n -62)||Control group (n –
X2(df) p -value
|E.coli||74||19 (82.6)||55 (85.9)||
***- signicant at p<0.0001 $ – Yate’s corrected
should be used and three way connectors Central venous catheters (CVC) are should be avoided.12 These guidelines inserted in critically ill patients and essential to the modern practices. CVC enables hemodynamic monitoring, collection of blood samples, administration of life supporting medicines and total parenteral nutrition5 , 6 Central Venous catheter related bloodstream infections (CRBSIs) signicantly increases mortality, morbidity as well as treatment expenses worldwide and listed among signicant complications associated with CVC related to CVC and closed catheter access system (Luer access Split septum) have been published and are being practiced in all the developed nations. Prevention of CRBSI is a major concern in intensive care units across the globe. The present study was done to compare the efcacy of closed catheter access system (Luer access Split Septum) and open catheter access system (three way connector) for prevention of CRBSI among critically ill patients p l a c e m e n t . 1 1 – 1 3 C R B S I r e d u c t i o n admitted in ICUs and to evaluate interventions were focused on infection surveillance and insertion bundle, maintenance and removal i.e. injection ports should be cleaned with alcohol and needleless adaptors for injection ports bacteriological prole of CRBSI.
For the proper execution of the study design, all nurses working in ICUs were trained for optimal care of patient with CVC and prevention of CRBSI as they are the key health care personnels for patient care in ICUs. Hadaway 10reported that when nurses gain experience and overcome the challenges of using the closed access system, i.e needleless system, infection rates will be less.
In present study, it had been found that both negative bacteria, 26% have gram-positive bacteria and 12.3% have fungi. Lin etal18 (2015) also identied pathogens which caused CRBSI following bundle care, and found Gram-ve bacteria (38%) to be most common, and followed by gram +ve bacteria (34.7%) and Candida spp. he groups were homogenous in terms of(24%).Abirami4 identied Klebsiella socio-demographic status, site of insertion, clinical diagnosis, type of insertion as per X2test(p>0.05). Mean age was 54.3±18.0 in experimental group and 55.9±17.8 in control group. Both the groups had maximum male patients. Similar ndings p n e u m o n i a e a n d s t a p h y l o c o c c u s epidermidis in CVC tip culture. Vading et al19 2018 found the most common gram- negative species, Klebsiella pneumoniae (KP) and Escherichia coli (EC) that cause invasive infections. Many studies reported were also reported by Mallikarjun14 and similar ndings regarding presence of gram Patil etal 201115 that mean age of patient’s negative organisms but Bhavana 1 6 , were 51.25yrs and maximum patients were
Mozaffari etal20 and Gahlotetal reported male. Bhavna etal16 also reported that that Staphylococcus aureus , gram +ve patients having CRBSI belong to age group of 51-60yrs and mean age for males was 43.48 yrs and females 42.87out of which 70% was males and 30% was females
Present study revealed that there was highly organism were isolated, followed by Candida tropicalis. Templeton 200821 reported staphylococci coagulase negative with a rate of 28%.were isolated in CRBSI positive culture. Krishnan et al22 2010 signicant (X2-58.305, p value -0.0001) difference in blood culture among patients in both groups. In experimental group 37.1% patients have positive and 62.9% patients have sterile blood culture but in found Gram-positive cocci (27%) and gram-negative bacilli (56%) caused CRBSI. CRBSI proportion caused by Gram-negative bacilli was lower in western hospitals.control group all patients have positive
Rosenthal VD9 et al reported that CVC blood culture. Gram-ve bacilli E.Coli and Klebsiella pneumoniae was present in both groups in the same proportion thus non- signicant p-value 0.966 was calculated. It probably happened as gram-ve infections are very common in ICUs. Oberai et al 20167 also found Klebsiella pneumonia as common isolated bacteria in CRBSI and CVC colonization. Jia, Yu and Lu17 reported similar ndings of blood culture of CRBSI positive patients that 61.7% have gram- associated bacteremia during use of the closed system was signicantly lower than during the use of the open system. To ll the gap between knowledge and practice regarding CLABSI/CRBSI prevention, educational programmes should be imparted routinely and nurses should use evidence-based practices.23
Based upon above ndings it was concluded that open access system (Luer access system) had better efcacy in prevention of catheter associated blood stream infections as compared to open access system (three way connectors). Nurses should have strict compliance to infection control prevention bundles in intensive care units. Training of all health care personals should be mandatory to reduce health care associated infections and to improve prognosis of patients .It was recommended that hospitals administrators should ensure implementation of infection control protocols according to CDC guidelines and mandatory training of staff nurses to prevent CRBSI in ICUs. Closed catheter access system ( Luer access split septum) should be used for CVC as well for peripheral venous lines to reduce blood stream infections in hospital which possibly reduce morbidity, mortality and hospital cost of patients.
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